CHIASMA Partners from the Hanyang University in Seoul (Korea) have developed an innovative high-dimensional approach to reveal immunological and transcriptomic responses of multiple cell types at the single cell level in a complex heterogeneous cellular environment.
Their study assessing the immunological response of food additive E171 TiO2, described in a paper entitled ‘Single-cell RNA sequencing uncovers heterogenous immune cell responses upon exposure to food additive (E171) titanium dioxide’, male Sprague-Dawley rats were used to deliver E171 TiO2 (5 mg/kg bw per day) via non-invasive intratracheal instillation for 13 weeks. After the 4 weeks recovery period, 3 mL of blood samples from both treated and untreated groups were collected for scRNAseq analysis. Firstly, granulocyte G1 activated innate immune response through the upregulation of genes involved in pro-inflammatory cytokine mediated cytotoxicity.
https://doi.org/10.1186/s12951-024-03036-9).
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